Product

PCR related

Anti HS Taq-High
  • Anti HS PCR Premix(-dye)๋Š” Hot start PCR ๋ฐ˜์‘์— ์ ํ•ฉํ•˜๋„๋ก ์ œ์กฐ๋˜์–ด, ์ƒ์˜จ์—์„œ๋Š” ํ™œ์„ฑ์ด ์—†์œผ๋ฉฐ,
  • 95oC์—์„œ 10๋ถ„๊ฐ„ activation ๊ณผ์ •์„ ๊ฑฐ์ณ ํ™œ์„ฑ์„ ๋‚˜ํƒ€๋ƒ…๋‹ˆ๋‹ค.
  • DNA amplification์‹œ์— ๋†’์€ ๋ฏผ๊ฐ๋„๋ฅผ ์–ป์„ ์ˆ˜ ์žˆ์Šต๋‹ˆ๋‹ค.
  • ๊ณ ์ˆœ๋„๋กœ ์ •์ œ๋˜์–ด Noise๊ฐ€ ์ „ํ˜€ ์—†์œผ๋ฉฐ, ์ตœ์ ํ™”๋œ Buffer system์€ ์›ํ•˜๋Š” ๊ฒฐ๊ณผ๋ฌผ๋งŒ์„ ์–ป์„ ์ˆ˜ ์žˆ๋„๋กํ•ฉ๋‹ˆ๋‹ค.
    • Cat No. : AHS-101 , AHS-102, AHS-103
    • Store at -20โ„ƒ
    • Store at -20โ„ƒ
    • Components :
    • - Anti-HS Taq -High- ----- 500U x 1,3,5
    • - 10x PCR buffer ---------- 1ใŽ–ย ย  x 2
    • - 10mM dNTP ------------- 1ใŽ–ย ย  x 1
    • - Manual ------------------- 1
    Applications
  • Hot start PCR , Multiplex PCR , Real-time PCR , Large size PCR , TA-Cloning , One Step PCR
    • PCR condition ( to 20ใŽ• )
    • Template DNAใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€: 1~5ใŽ•
    • 10x Bufferใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€: 2ใŽ•
    • 10mM dNTP mixใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ ใ€€ใ€€ใ€€ใ€€ใ€€: 0.5~2.0ใŽ•
    • Forward Pimer (10 pmole)ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€: X ใŽ•
    • Reverse Pimer (10 pmole)ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€: X ใŽ•
    • D.Wใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ : Up to 20ใŽ•
    • Anti HS Taq โ€“High- ใ€€ใ€€ใ€€ใ€€ ใ€€ ใ€€ใ€€ใ€€ใ€€ : 1ใŽ•
    • Total Vol.ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ : 20ใŽ•
    • Cycling conditions
    • Initial Denaturationใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 94~95โ„ƒ : 10 min
    • Denaturationใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€94โ„ƒ : 30 sec
    • Annealingใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 50~60โ„ƒ : 30 sec
    • Extensionใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 72โ„ƒ : 30~60 sec
    • DNA Anplificationใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€25~35 cycles
    • Final Extensionใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€72โ„ƒ : 5 min
    • Storeใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 4โ„ƒ : โˆž

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