Product

PCR related

Anti HS Taq Premix (2X)
  • Anti HS Taq Premix(2X)๋Š” Hot Start PCR ๋ฐ˜์‘์— ์ ํ•ฉํ•˜๋„๋ก ์ œ์กฐ๋˜์–ด, ์ƒ์˜จ์—์„œ๋Š” ํ™œ์„ฑ์ด ์—†์œผ๋ฉฐ, 95oC์—์„œ 10๋ถ„๊ฐ„ activation ๊ณผ์ •์„ ๊ฑฐ์ณ
  • ํ™œ์„ฑ์„ ๋‚˜ํƒ€๋ƒ…๋‹ˆ๋‹ค.
  • DNA amplification์‹œ์— ๋†’์€ ๋ฏผ๊ฐ๋„๋ฅผ ์–ป์„ ์ˆ˜ ์žˆ์Šต๋‹ˆ๋‹ค.
  • ๊ณ ์ˆœ๋„๋กœ ์ •์ œ๋˜์–ด Noise๊ฐ€ ์ „ํ˜€ ์—†์œผ๋ฉฐ, ์ตœ์ ํ™”๋œ Buffer system์€ ์›ํ•˜๋Š” ๊ฒฐ๊ณผ๋ฌผ๋งŒ์„ ์–ป์„ ์ˆ˜ ์žˆ๋„๋ก ํ•ฉ๋‹ˆ๋‹ค.
    • Cat No. : AHS-201 , AHS-202, AHS-203
    • Store at -20โ„ƒ
    • Components :
    • - Anti-HS Taq Premix ----- 1ใŽ– x 1,3,5
    • - Manual ------------------- 1
    Applications
  • Hot start PCR , Multiplex PCR , Real-time PCR , Large size PCR , TA-Cloning , One Step PCR
    • PCR condition ( to 20ใŽ• )
    • Template DNAใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ : Variable
    • Anti HS Taq Premix (2X)ใ€€ใ€€ ใ€€ : 10ใŽ•
    • Forward Pimer (10 pmole) ใ€€ ใ€€: 0.5~2.0ใŽ•
    • Reverse Pimer (10 pmole) ใ€€ ใ€€ : 0.5~2.0ใŽ•
    • D.W ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€: Up to 20ใŽ•
    • Total Vol.ใ€€ ใ€€ใ€€ใ€€ใ€€ใ€€ ใ€€ใ€€ใ€€ใ€€ : 20ใŽ•
    • Cycling conditions
    • Initial Denaturationใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 94~95โ„ƒ : 10 min
    • Denaturationใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€94โ„ƒ : 30 sec
    • Annealingใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 50~60โ„ƒ : 30 sec
    • Extensionใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 72โ„ƒ : 30~60 sec
    • DNA Anplificationใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€25~35 cycles
    • Final Extensionใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€72โ„ƒ : 5 min
    • Storeใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 4โ„ƒ : โˆž
    Anti HS Taq Premix (8-strip)
  • Anti HS Taq Premix(2X)๋Š” Hot Start PCR ๋ฐ˜์‘์— ์ ํ•ฉํ•˜๋„๋ก ์ œ์กฐ๋˜์–ด, ์ƒ์˜จ์—์„œ๋Š” ํ™œ์„ฑ์ด ์—†์œผ๋ฉฐ, 95oC์—์„œ 10๋ถ„๊ฐ„ activation ๊ณผ์ •์„
  • ๊ฑฐ์ณ ํ™œ์„ฑ์„ ๋‚˜ํƒ€๋ƒ…๋‹ˆ๋‹ค.
  • DNA amplification์‹œ์— ๋†’์€ ๋ฏผ๊ฐ๋„๋ฅผ ์–ป์„ ์ˆ˜ ์žˆ์Šต๋‹ˆ๋‹ค.
  • ๊ณ ์ˆœ๋„๋กœ ์ •์ œ๋˜์–ด Noise๊ฐ€ ์ „ํ˜€ ์—†์œผ๋ฉฐ, ์ตœ์ ํ™”๋œ Buffer system์€ ์›ํ•˜๋Š” ๊ฒฐ๊ณผ๋ฌผ๋งŒ์„ ์–ป์„ ์ˆ˜ ์žˆ๋„๋ก ํ•ฉ๋‹ˆ๋‹ค.
    • Cat No. : AHS-301 , AHS-302, AHS-303
    • Store at -20โ„ƒ
    • Components :
    • - Anti-HS Taq Premix ----- 96T x 1,3,5
    • - Manual ------------------- 1
    Applications
  • Hot start PCR , Multiplex PCR , Real-time PCR , Large size PCR , TA-Cloning , One Step PCR
    • PCR condition ( to 20ใŽ• )
    • Template DNAใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ : Variable
    • Anti HS Taq Premix (2X)ใ€€ใ€€ ใ€€ : 10ใŽ•
    • Forward Pimer (10 pmole) ใ€€ ใ€€: 0.5~2.0ใŽ•
    • Reverse Pimer (10 pmole) ใ€€ ใ€€ : 0.5~2.0ใŽ•
    • D.W ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€: Up to 20ใŽ•
    • Total Vol.ใ€€ ใ€€ใ€€ใ€€ใ€€ใ€€ ใ€€ใ€€ใ€€ใ€€ : 20ใŽ•
    • Cycling conditions
    • Initial Denaturationใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 94~95โ„ƒ : 10 min
    • Denaturationใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€94โ„ƒ : 30 sec
    • Annealingใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 50~60โ„ƒ : 30 sec
    • Extensionใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 72โ„ƒ : 30~60 sec
    • DNA Anplificationใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€25~35 cycles
    • Final Extensionใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€72โ„ƒ : 5 min
    • Storeใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 4โ„ƒ : โˆž