Product

PCR related

Pro-Taq Master Mix
  • Pro-Hot Start PCR Master Mix๋Š” Hot start PCR ๋ฐ˜์‘์— ์ ํ•ฉํ•˜๋„๋ก ์ œ์กฐ๋˜์–ด, ์ƒ์˜จ์—์„œ๋Š” ํ™œ์„ฑ์ด ์—†์œผ๋ฉฐ, 95oC์—์„œ 10๋ถ„๊ฐ„ activation ๊ณผ์ •์„
  • ๊ฑฐ์ณ ํ™œ์„ฑ์„ ๋‚˜ํƒ€๋ƒ…๋‹ˆ๋‹ค.
  • DNA amplification์‹œ์— ๋†’์€ ๋ฏผ๊ฐ๋„๋ฅผ ์–ป์„ ์ˆ˜ ์žˆ์Šต๋‹ˆ๋‹ค.
  • ๊ณ ์ˆœ๋„๋กœ ์ •์ œ๋˜์–ด Noise๊ฐ€ ์ „ํ˜€ ์—†์œผ๋ฉฐ, ์ตœ์ ํ™”๋œ Buffer system์€ ์›ํ•˜๋Š” ๊ฒฐ๊ณผ๋ฌผ๋งŒ์„ ์–ป์„ ์ˆ˜ ์žˆ๋„๋ก ํ•ฉ๋‹ˆ๋‹ค.
    • Cat No. : ST-MTP-01
    • Store at -20โ„ƒ
    • Components :
    • - Pro-Taq Master Mix---------- 500U x 4
    • - Manual ------------------- 1
    Applications
  • Hot start PCR , Multiplex PCR , Real-time PCR , Large size PCR , TA-Cloning , One Step PCR
    • PCR condition ( to 20ใŽ• )
    • Template DNAใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ : 1~5ใŽ•
    • Forward Pimer (10 pmole) ใ€€ ใ€€: X ใŽ•
    • Reverse Pimer (10 pmole) ใ€€ ใ€€ : X ใŽ•
    • D.W ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€: Up to 50ใŽ•
    • Pro Taq Master Mixใ€€ใ€€ใ€€ใ€€ใ€€ใ€€: 25ใŽ•
    • Total Vol.ใ€€ ใ€€ใ€€ใ€€ใ€€ใ€€ ใ€€ใ€€ใ€€ใ€€ : 50ใŽ•
    • Cycling conditions
    • Initial Denaturationใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 94~95โ„ƒ : 10 min
    • Denaturationใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€94โ„ƒ : 30 sec
    • Annealingใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 50~60โ„ƒ : 30 sec
    • Extensionใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 72โ„ƒ : 30~60 sec
    • DNA Anplificationใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€25~35 cycles
    • Final Extensionใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€72โ„ƒ : 5 min
    • Storeใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 4โ„ƒ : โˆž
    Pro-Taq DNA Polymerase
  • Pro-Taq DNA Polymerase๋Š” ~10kb ํฌ๊ธฐ๊นŒ์ง€ ์ฆํญ๊ฐ€๋Šฅํ•˜๋ฉฐ, proofreading ๊ธฐ๋Šฅ์ด ์ถ”๊ฐ€๋˜์–ด ๋‚ฎ์€ error์œจ๋กœ ๋‹ค์–‘ํ•œ PCR๋ฐ˜์‘์— ํšจ๊ณผ์ ์œผ๋กœ
  • ์ด์šฉํ•  ์ˆ˜ ์žˆ์Šต๋‹ˆ๋‹ค.
  • ๊ณ ์ˆœ๋„๋กœ ์ •์ œ๋˜์–ด noise๊ฐ€ ์ „ํ˜€ ์—†์œผ๋ฉฐ, ์ตœ์ ํ™”๋œ buffer system์€ ์›ํ•˜๋Š” ๊ฒฐ๊ณผ๋ฌผ๋งŒ์„ ์–ป์„ ์ˆ˜ ์žˆ๋„๋ก ํ•ฉ๋‹ˆ๋‹ค.
  • 1kb ์ฆํญ์— 30sec. ์ •๋„ ์†Œ์š”๋ฉ๋‹ˆ๋‹ค.
    • Cat No. : ST-TP-01
    • Store at -20โ„ƒ
    • Components :
    • - Pro-Taq DNA Polymerase----- 500U x 1
    • - 10x PCR buffer ---------------- 1ใŽ– x 2
    • - 2.5mM dNTP ------------------ 1ใŽ– x 1
    • - Manual ------------------------- 1
    Applications
  • Hot start PCR , Multiplex PCR , Real-time PCR , Large size PCR , TA-Cloning , One Step PCR
    • PCR condition ( to 20ใŽ• )
    • Template DNAใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ : Variable
    • 10x Bufferใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ : 2ใŽ•
    • 2.5mM dNTP mixใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€: 0.5~2.0ใŽ•
    • Forward Pimer (10 pmole) ใ€€ ใ€€: X ใŽ•
    • Reverse Pimer (10 pmole) ใ€€ ใ€€ : X ใŽ•
    • D.W ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€: Up to 20ใŽ•
    • Pro-Taq DNA Polymerase ใ€€ใ€€ใ€€: 0.2~0.5ใŽ•
    • Total Vol.ใ€€ ใ€€ใ€€ใ€€ใ€€ใ€€ ใ€€ใ€€ใ€€ใ€€ : 20ใŽ•
    • Cycling conditions
    • Initial Denaturationใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 94~95โ„ƒ : 10 min
    • Denaturationใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€94โ„ƒ : 30 sec
    • Annealingใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 50~60โ„ƒ : 30 sec
    • Extensionใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 72โ„ƒ : 30~60 sec
    • DNA Anplificationใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€25~35 cycles
    • Final Extensionใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€72โ„ƒ : 5 min
    • Storeใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 4โ„ƒ : โˆž
    Pro-Taq PCR Master Mix
  • Pro-Taq DNA Polymerase๋Š” ~10kb ํฌ๊ธฐ๊นŒ์ง€ ์ฆํญ๊ฐ€๋Šฅํ•˜๋ฉฐ, proofreading ๊ธฐ๋Šฅ์ด ์ถ”๊ฐ€๋˜์–ด ๋‚ฎ์€ error์œจ๋กœ ๋‹ค์–‘ํ•œ PCR๋ฐ˜์‘์— ํšจ๊ณผ์ ์œผ๋กœ
  • ์ด์šฉํ•  ์ˆ˜ ์žˆ์Šต๋‹ˆ๋‹ค.
  • ๊ณ ์ˆœ๋„๋กœ ์ •์ œ๋˜์–ด noise๊ฐ€ ์ „ํ˜€ ์—†์œผ๋ฉฐ, ์ตœ์ ํ™”๋œ buffer system์€ ์›ํ•˜๋Š” ๊ฒฐ๊ณผ๋ฌผ๋งŒ์„ ์–ป์„ ์ˆ˜ ์žˆ๋„๋ก ํ•ฉ๋‹ˆ๋‹ค.
  • 1kb ์ฆํญ์— 30sec. ์ •๋„ ์†Œ์š”๋ฉ๋‹ˆ๋‹ค.
    • Cat No. : ST-MTP-96
    • Store at -20โ„ƒ
    • Components :
    • - Pro-Taq PCR Master Mix ------- 96T x 5
    • - Manual ------------------------- 1
    Applications
  • Hot start PCR , Multiplex PCR , Real-time PCR , Large size PCR , TA-Cloning , One Step PCR
    • PCR condition ( to 20ใŽ• )
    • Template DNAใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ : X ใŽ•
    • Forward Pimer (10 pmole) ใ€€ ใ€€: X ใŽ•
    • Reverse Pimer (10 pmole) ใ€€ ใ€€ : X ใŽ•
    • D.W ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€: Up to 20ใŽ•
    • Pro-Taq DNA Polymerase ใ€€ใ€€ใ€€: ๋™๊ฒฐ๊ฑด์กฐ
    • Total Vol.ใ€€ ใ€€ใ€€ใ€€ใ€€ใ€€ ใ€€ใ€€ใ€€ใ€€ : 20ใŽ•
    • Cycling conditions
    • Initial Denaturationใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 94~95โ„ƒ : 10 min
    • Denaturationใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€94โ„ƒ : 30 sec
    • Annealingใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 50~60โ„ƒ : 30 sec
    • Extensionใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 72โ„ƒ : 30~60 sec
    • DNA Anplificationใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€25~35 cycles
    • Final Extensionใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€72โ„ƒ : 5 min
    • Storeใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 4โ„ƒ : โˆž
    Pro-Pfu DNA Polymerase
  • Pro-Pfu DNA Polymerase๋Š” ~10kb ํฌ๊ธฐ๊นŒ์ง€ ์ฆํญ๊ฐ€๋Šฅํ•˜๋ฉฐ, proofreading ๊ธฐ๋Šฅ์— ์˜ํ•œ ์ •๋ฐ€ํ•œ PCR๋ฐ˜์‘์— ํšจ๊ณผ์ ์œผ๋กœ
  • ์ด์šฉํ•  ์ˆ˜ ์žˆ์Šต๋‹ˆ๋‹ค.
  • ๊ณ ์ˆœ๋„๋กœ ์ •์ œ๋˜์–ด noise๊ฐ€ ์ „ํ˜€ ์—†์œผ๋ฉฐ, ์ตœ์ ํ™”๋œ buffer system์€ ์›ํ•˜๋Š” ๊ฒฐ๊ณผ๋ฌผ๋งŒ์„ ์–ป์„ ์ˆ˜ ์žˆ๋„๋ก ํ•ฉ๋‹ˆ๋‹ค.
  • 1kb ์ฆํญ์— 30sec. ์ •๋„ ์†Œ์š”๋ฉ๋‹ˆ๋‹ค.
    • Cat No. : ST-PF-01
    • Store at -20โ„ƒ
    • Components :
    • - Pro-Pfu DNA Polymerase----- 500U x 1
    • - 10x PCR buffer ---------------- 1ใŽ– x 2
    • - 2.5mM dNTP ------------------ 1ใŽ– x 1
    • - Manual ------------------------- 1
    Applications
  • Hot start PCR , Multiplex PCR , Real-time PCR , Large size PCR , TA-Cloning , One Step PCR
    • PCR condition ( to 20ใŽ• )
    • Template DNAใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ : 1~5ใŽ•
    • 10x Bufferใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ : 5ใŽ•
    • 2.5mM dNTP mixใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€: 4ใŽ•
    • Forward Pimer (10 pmole) ใ€€ ใ€€: 0.5~5ใŽ•
    • Reverse Pimer (10 pmole) ใ€€ ใ€€ : 0.5~5ใŽ•
    • D.W ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€: Up to 50ใŽ•
    • Pro-Taq DNA Polymerase ใ€€ใ€€ใ€€: 0.2~0.5ใŽ•
    • Total Vol.ใ€€ ใ€€ใ€€ใ€€ใ€€ใ€€ ใ€€ใ€€ใ€€ใ€€ : 50ใŽ•
    • Cycling conditions
    • Initial Denaturationใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 94~95โ„ƒ : 10 min
    • Denaturationใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€94โ„ƒ : 30 sec
    • Annealingใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 50~60โ„ƒ : 30 sec
    • Extensionใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 72โ„ƒ : 30~60 sec
    • DNA Anplificationใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€25~35 cycles
    • Final Extensionใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€72โ„ƒ : 5 min
    • Storeใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 4โ„ƒ : โˆž
    Pro-Hot Start Taq Polymerase, 500U
  • Pro-Hot Start Taq Polymerase๋Š” Hot start PCR ๋ฐ˜์‘์— ์ ํ•ฉํ•˜๋„๋ก ์ œ์กฐ๋˜์–ด, ์ƒ์˜จ์—์„œ๋Š” ํ™œ์„ฑ์ด ์—†์œผ๋ฉฐ, 95oC์—์„œ 15๋ถ„๊ฐ„ activation ๊ณผ์ •์„
  • ๊ฑฐ์ณ ํ™œ์„ฑ์„ ๋‚˜ํƒ€๋ƒ…๋‹ˆ๋‹ค.
  • ๊ณ ์ˆœ๋„๋กœ ์ •์ œ๋˜์–ด noise๊ฐ€ ์ „ํ˜€ ์—†์œผ๋ฉฐ, ์ตœ์ ํ™”๋œ buffer system์€ ์›ํ•˜๋Š” ๊ฒฐ๊ณผ๋ฌผ๋งŒ์„ ์–ป์„ ์ˆ˜ ์žˆ๋„๋ก ํ•ฉ๋‹ˆ๋‹ค.
  • DNA amplification ์‹œ์— ๋†’์€ ๋ฏผ๊ฐ๋„๋ฅผ ์–ป์„ ์ˆ˜ ์žˆ์Šต๋‹ˆ๋‹ค.
    • Cat No. : ST-HT-01
    • Store at -20โ„ƒ
    • Components :
    • - Pro-Hot Start Taq Polymerase - 500U x 1
    • - 10x PCR buffer ------------------ 1ใŽ– x 2
    • - 2.5mM dNTP -------------------- 1ใŽ– x 1
    • - Manual ------------------------- 1
    Applications
  • Hot start PCR , Multiplex PCR , Real-time PCR , Large size PCR , TA-Cloning , One Step PCR
    • PCR condition ( to 20ใŽ• )
    • Template DNAใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ : 1~5ใŽ•
    • 10x Bufferใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ : 5ใŽ•
    • 2.5mM dNTP mixใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€: 4ใŽ•
    • Forward Pimer (10 pmole) ใ€€ ใ€€: 0.5~5ใŽ•
    • Reverse Pimer (10 pmole) ใ€€ ใ€€ : 0.5~5ใŽ•
    • D.W ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€: Up to 50ใŽ•
    • Pro-Taq DNA Polymerase ใ€€ใ€€ใ€€: 1ใŽ•
    • Total Vol.ใ€€ ใ€€ใ€€ใ€€ใ€€ใ€€ ใ€€ใ€€ใ€€ใ€€ : 50ใŽ•
    • Cycling conditions
    • Initial Denaturationใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 94~95โ„ƒ : 10 min
    • Denaturationใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€94โ„ƒ : 30 sec
    • Annealingใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 50~60โ„ƒ : 30 sec
    • Extensionใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 72โ„ƒ : 30~60 sec
    • DNA Anplificationใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€25~35 cycles
    • Final Extensionใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€72โ„ƒ : 5 min
    • Storeใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 4โ„ƒ : โˆž
    Pro-Hot Start PCR Master Mix
  • 20ul ๋ฐ˜์‘ ๊ธฐ์ค€์œผ๋กœ ์ œ์กฐ๋˜์—ˆ์œผ๋ฉฐ, 95โ„ƒ์—์„œ 15๋ถ„๊ฐ„ activation ๊ณผ์ •์„ ๊ฑฐ์ณ ํ™œ์„ฑ์„ ๋‚˜ํƒ€๋ƒ…๋‹ˆ๋‹ค.
  • Enzyme, buffer, dNTP, stabilizer, loading buffer๊ฐ€ ํ˜ผํ•ฉ๋˜์–ด ์žˆ์–ด Primer์™€ Template DNA์˜ ์ฒจ๊ฐ€๋งŒ์œผ๋กœPCR ๋ฐ˜์‘์„ ๊ฐ„ํŽธํ•˜๊ฒŒ
  • ์ˆ˜ํ–‰ ํ•  ์ˆ˜ ์žˆ์Šต๋‹ˆ๋‹ค.
  • Proof-reading ๊ธฐ๋Šฅ์ด ์žˆ์–ด ๋‚ฎ์€ error rate ๋ฐ 10kb๊นŒ์ง€ ์ฆํญ์ด ๊ฐ€๋Šฅํ•ฉ๋‹ˆ๋‹ค.
    • Cat No. : ST-MHP-96
    • Store at -20โ„ƒ
    • Components :
    • - Pro-Hot Start PCR Master Mix - 96T x 1
    • - Manual ------------------------- 1
    Applications
  • Hot start PCR , Multiplex PCR , Real-time PCR , Large size PCR , TA-Cloning , One Step PCR
    • PCR condition ( to 20ใŽ• )
    • Template DNAใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ : XใŽ•
    • Forward Pimer (10 pmole) ใ€€ ใ€€: 0.5~1ใŽ•
    • Reverse Pimer (10 pmole) ใ€€ ใ€€ : 0.5~1ใŽ•
    • D.W ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€: Up to 20ใŽ•
    • Total Vol.ใ€€ ใ€€ใ€€ใ€€ใ€€ใ€€ ใ€€ใ€€ใ€€ใ€€ : 20ใŽ•
    • Cycling conditions
    • Initial Denaturationใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 94~95โ„ƒ : 10 min
    • Denaturationใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€94โ„ƒ : 30 sec
    • Annealingใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 50~60โ„ƒ : 30 sec
    • Extensionใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 72โ„ƒ : 30~60 sec
    • DNA Anplificationใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€25~35 cycles
    • Final Extensionใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€72โ„ƒ : 5 min
    • Storeใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ใ€€ 4โ„ƒ : โˆž